《植物生理学报》 2009, 45(4): 318-322

转星星草金属硫蛋白基因烟草的获得及其抗重金属镉能力分析

王雷, 曲跃军, 杜人杰, 吴丽丽, 王玉成, 姜廷波*

东北林业大学, 林木遗传育种与生物技术教育部重点实验室, 哈尔滨150040

通信作者：姜廷波；E-mail: tbj iang@yahoo.com；Tel: 0451-82190607

摘　要：

用农杆菌介导法将星星草金属硫蛋白基因(MT)转化烟草, PCR 及PCR-Southern 检测的结果表明, 该基因已导入烟草 中。Real-Time PCR 检测显示, 该基因在转基因后代中的转录水平高于非转基因植株。Cd2+ 胁迫下, 转基因植株能够正常 生长, 鲜重、株高、叶绿素含量、Cd2+ 含量和 SOD 活性均高于非转基因植株, 表明 MT 基因的过量表达可提高转基因烟草 的抗 Cd2+ 能力。

关键词：烟草; 金属硫蛋白; 转基因

收稿：2008-12-01   修定：2009-02-06

资助：黑龙江省重点科技攻关项目(GB06B303-5)

Identification of MT Overexpression in Tobacco and Analysis of Cd²⁺ Resistance in Transgenic Plants

WANG Lei, QU Yue-Jun, DU Ren-Jie, WU Li-Li, WANG Yu-Cheng, JIANG Ting-Bo*

Key Laboratory of Forest Tree Genetic Improvement and Biotechnology of Ministry of Education, Northest Forest University, Harbin 150040, China

Corresponding author: JIANG Ting-Bo; E-mail: tbj iang@yahoo.com; Tel: 0451-82190607

Abstract:

The metallothionein gene (MT) from Puccinellia tenuiflora was transformed into tobacco by Agrobacterium tumefaciens. The results of PCR and PCR-Southern hybridization showed that the transformed MT gene expressed in tobacco. Real-Time PCR demonstrated that the transcript level of MT gene in T₁ generation was higher than that in wild plants. With the treatment of Cd²⁺, the transgenic tobacco plants could grow normally. Fresh weight, plant high, chlorophyll content, Cd²⁺ content and activity of SOD were all higher than those in wild plants. The overexpression of MT gene enhanced the resistance to Cd²⁺ in transgenic tobacco plants.

Key words: tobacco; metallothionein; genetic transformation